| Name | Rank | Date of Muster Into Service | Remarks |
| David H. Williams | Colonel | July 3, '61 | Discharged February 14, 1863 |
| Isaac C. Bassett | do | August 24,'61 | Promoted from Captain Co. K to Maj. Feb. 7, '63 to Col. May 3, '63 to Bvt. Brig. Gen. Dec. 12, '64 absent, in arrest, at mus. out of regiment |
| Frank Vallee | Lt. Col. | July 21, '61 | Discharged on surgeon's certificate Jan. 8, '63 |
| John M. Wetherill | do | Aug. 24, '61 | Promoted from Major, June 28, 1863 mustered out Sept. 16, 1864 expiration of term |
| James R. Neiler | do | Aug. 24, '61 | Promoted to Brevet Colonel April 6, 1863 Mustered out with regiment July 13, 1863 |
| William Clark | Major | Aug. 14, '61 | Promoted from Capt. Co. E to Major Feb. 28, '65 to Bvt. Lt. Col. April 6, 1865 Ab. at muster out |
| Matthew C. Grier | Adj't | Aug. 14, '61 | Promoted from 1st Lieutenant company G discharged on Surgeon's certificate Feb. 6, 1863 |
| Owen Tompkins | do | Aug. 22, '61 | Promoted to Adjutant Feb. 6, 1863 Mustered out Sept. 16, 1864 expiration of term |
| B. Theo. Northrup | do | Sept. 6, '61 | Promoted to Adj't. Oct. 6, '64 to Bvt. Capt. April 6, 1865 mustered out with regiment July 13, 1865 |
| George Whiting | Q.M. | Jan. 1, '62 | Resigned January 24, 1862 |
| James B. Grier | do | Aug. 14, '61 | Promoted from 1st Lieutenant Co. G Feb. 1, 1862 Killed at Malvern Hill, Va. July 1, 1862 |
| Wallace B. Todd | do | Aug. 27, '61 | Promoted from 2nd Lt. Co. K May 4, 1862 Mustered out Sept. 16, 1864 expiration of term |
| Hiram P. Callow | do | Aug. 20, '61 | Promoted from 1st Lt. Co. D Oct. 6, 1864 mustered out with regiment July 13, 1865 Vet. |
| Orlando B. Griffith | do | Aug. 20, '61 | Promoted to Captain Co. II Jan. 1, 1862 |
| David D. Clark | Surg | July 22, '61 | Discharged on Surgeon's certificate Nov. 21, '62 |
| Louis M. Emmanuel | do | Aug. 15, '61 | Promoted from Asst. Surg. discharged Dec. 12, 1862 |
| John P. Richardson | do | March 23, '63 | Pr. from Asst. Surg. Oct 7, '64 resigned June 10, 1865 |
| Joseph Thomas | do | July 6, '65 | Mustered out with regiment July 13, 1865 |
| Richard Foote | Asst. Surg | Aug. 6, '62 | Resigned January 15, 1864 |
| James Millar | do | Jan. 14, '65 | Mustered out with regiment July 13, 1865 |
| Henry K. Brous | do | Feb. 24, '65 | Mustered out with regiment July 13, 1865 |
| Robert W. Oliver | Chap'n | Aug. 13, '61 | Discharged December 9, 1862 |
| Albert Burton | Sr. Maj. | Promoted to 2nd Lieutenant Co. K. Aug. 10, 1862 | |
| Charles K. Beecher | do | Aug. 20, '61 | Promoted to 1st Lieutenant Co. B Jan.13, 1865 Vet. |
| James Toomey | do | Feb. 23, '64 | Promoted from Sgt. Co. G Jan 16, '65 mustered out with regiment July 13, 1865 Vet. |
| Jos. W. P. Roberts | Q.M.Sr. | Aug. 29, '61 | Prmoted to 2nd Lt. Co. G Oct. 16, 1864 |
| B. Franklin Jenkins | do | Dec. 26, '63 | Mustered out with regiment July 13, 1865 Vet. |
| J. Wesley Myers | Com. Sr. | Aug. 20, '61 | Promoted to 1st Lieutenant Co. K Aug. 10, 1862 |
| Robert W. Patrick | do | Aug. 20, '61 | Promoted to 2nd Lt. Company E Nov. 9, 1962 |
| Mahlon H. Mitchner | do | Aug. 12, '61 | Promoted from Sgt. Co. C Jan. 27, '63 Mustered out with company July 13, 1865 Vet. |
| William Lancaster | Hos. St. | Aug. 12, '61 | Mustered out Sept. 16, 1864 expiration of term. |
| Fred'k A. Neville | do | Sept. 1, '61 | Mustered out with regiment July 13, 1865 Vet. |
| George Douglass | Pl. Muc. | Sept. 12, '61 | Discharged by General Order July 1862 |
| George W. Beckel | do | July 23, '61 | Promoted from Pvt. Co. A Aug 10, '62 Mustered out Sept. 16, '64 expiration of term. |
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