| Name | Rank | Date of Muster into Service | Term Years | Remarks |
| Horatio G. Sickel | Col | May 27, '61 | 3 | Promoted from Capt. company K, to Col. June 21, 1861-mus.out with regiment,June 17, 1864 |
| Wm. S. Thompson | Lt.Col. | July 28, '61 | 3 | Resigned July 9, 1862 |
| John Clark | do | May 31, '61 | 3 | Promoted from Capt. company E, to Lt.Colonel, Aug. 1, 1862-mus.outwith reg., June 17, 1864 |
| Rich. H. Woolworth | Major | June 1, '61 | 3 | Promoted to Lt Col. 33d reg. P.V., June 1, 1862 |
| William Briner | do | June 7, '61 | 3 | Promoted from Capt. company D, to Major, Aug. 1, 1862-mus.out withreg. June 17, 1864 |
| Albert H. Jamison | Adj | June 11, '61 | 3 | Discharged on Surgeon's certificate, Sept. 30, '62 |
| Harry S. Jones | do | July 28, '61 | 3 | Promoted from Sgt. Major, to 2d Lt. company F, July 29, 1862-to Adj.,October 1, 1862-mustered out with regiment, June 17, 1864 |
| Frank'n S. Bickley | Q.M. | June 7, '61 | 3 | Discharged on Surgeon's certificate, Nov. 13, '61 |
| Strickland Yardley | do | June 10, '61 | 3 | Promoted to A.Q.M., U.S. vol., Aug. 10, 1863 |
| Levi S. Boyer | do | July 28, '61 | 3 | Promoted to Q.M.Sgt., October 1, 1861-to reg. Q.M., December 14, 1863-musteredout with regiment, June 17, 1864 |
| James Collins | Surg. | July 28, '61 | 3 | Tr. to U.S. Vol, as Ast. Surg., April 14, 1864 |
| Geo. L. Pancoast | As.Sur. | July 28, '61 | 3 | Promoted to Surgeon U.S. Vol,Oct. 20, 1861 |
| Henry S. Colston | do | Nov. 2, '61 | 3 | Promoted to Surg. 81st reg.P.V., Aug. 19, 1862 |
| Samuel S. Orr | do | July 13, '62 | 3 | Resigned March 13, 1863 |
| George J. Rice | do | Aug. 2, '62 | 3 | Resigned January 17, 1863 |
| Stanton A. Welch | do | Jan. 29, '63 | 3 | Mustered out with regiment June 17, 1864 |
| John P. Birchfield | do | May 29, 63 | 3 | Left, at muster out, in charge of veterans from 32d and 33d regiments,P.V. |
| James M. Hoffman | do | Nov. 18, '61 | 3 | Not on muster-out roll. |
| William H. Leake | Chap'n | June 5, '61 | 3 | Resigned February 28, 1862 |
| George H. Frear | do | April 8, '62 | 3 | Resigned July 9, 1862 |
| John J. Pomeroy | do | Sept. 10, '62 | 3 | Mustered out with regiment, June 17, 1864 |
| William P. Smith | Sr.Maj | May 27, '61 | 3 | Promoted to Sgt. Major, March 1, 1863-mustered out with regiment, June17, 1864 |
| George M. Rhone | do | July 17, '61 | 3 | Promoted to Sgt.Major, August 1, 1862-to 2d Lieutenant company B, March1, 1863 |
| Irvine C. Wright | Q.M.S. | May 29, '61 | 3 | Promoted to Com.Sgt., November 1, 1862-to Q.M.Sgt., March 13, 1864-musteredout with regiment, June 17, 1864 |
Albert Briner | do | July 28, '61 | 3 | Discharged on Surgeon's certificate, Sept. 30, '61 |
| D.C. Eppeheimer | Com.Sr. | June 7, '61 | 3 | Promoted to ComSgt., March 1, 1864-mustered out with company, June17, 1864 |
| Duval Doran | do | May 29, '61 | 3 | Promoted to Com.Sgt., June 1, 1861-discharged on Surgeon's certificate,October 22, 1862 |
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