| Name | Rank | Date of Muster into Service | term years | Remarks |
| Joseph Thomas | Capt. | June 18, '61 | 3 | Resigned July 7, 1862 |
| B.F. Fisher | do | June 18, '61 | 3 | Detached to Signal Corps. Aug. 27, '61 pr. from 1st Lieutenant to Captain, July 7, 1862 mustered out with company, June 17, 1864 |
| J.B. Bartholomew | 1st Lt. | June 18, '61 | 3 | Pr. from 1st Sgt. to 2d Lt., Jan. 1, '62 to 1st Lt. July 7, '62 mustered out with Co., June 17, 1864 |
| Nelson Applebach | 2d Lt. | June 18, '61 | 3 | Resigned December 9, 1861 |
| William M'Carty | do | June 18, '61 | 3 | Pr. from 1st Sgt. to 2d. Lt., June 7, '62 detached May 30, 1864 to command 61st company Vets., 3d Brigade, 2d Division, Dep't West Virginia. |
| Andrew J. Adrian | 1st Sgt. | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| Ephraim Case | do | June 18, '61 | 3 | Killed at Antietam, September 17, 1862 |
| J.H.F.A.Christern | Serg't | June 18, '61 | 3 | Mustered out with company June 17, 1864 |
| Jesse Keller | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Isaac E. Lewis | do | June 18, '61 | 3 | Died of wounds at Alexandria, September 24, 1862 - grave 1,508. |
| George W. Vaux | do | June 18, '61 | 3 | Wd. and prisoner at Cloyd Mountain, May 9, '64 |
| Hugh O'Connell | Corp. | June 29, '61 | 3 | Mustered out with company, June 17, 1864 |
| Augustus Neiffer | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Marshall Darling | do | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| Dan'l Frankenfield | do | June 18, '61 | 3 | Discharged and re-enlisted in battery C, 5th United States Artillery, November 29, 1862. |
| Nathan Hauch | do | June 29, '61 | 3 | Discharged on Surgeons certificate Jan. 20, '63 |
| Rozell M. Gaylord | do | June 22, '61 | 3 | Transferred to Vet.Res.Corps date unknown |
| Jackson Bachman | do | June 18, '61 | 3 | Died at Fortress Monroe, September 21, 1862 |
| Israel Long | do | June 29, '61 | 3 | Died of wds.rec. at Cloyd Mountain, May 9, '64 |
| Harvey Alabach | do | June 18, '61 | 3 | Killed at Charles City Cross Roads, June 30, '62 |
| Levi Frey | do | June 29, '61 | 3 | Deserted February 6, 1863 |
| C. Kluman | Muc | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| William Burnes | do | June 29, '61 | 3 | Transferred to company I, July 1, 1861 |
| Albright, John | Private | June 18, '61 | 3 | Discharged re-enlisted in battery C, 5th United States Artillery, November 24, 1862 |
| Brey, Adam S. | do | June 18, '61 | 3 | Absent, sick, at muster out. |
| Beidlingmoyer, C. | do | July 15, '61 | 3 | Absent, sick at muster out. |
| Boone, Allen | do | July 8, '61 | 3 | Discharged on Surgeons certificate, Nov. 21, '62 |
| Burnes, Lewis | do | April 10, '62 | 3 | Discharged on Surgeons certificate, Feb. 24, '63 |
| Bushnell, Sam'l A. | do | July 29, '61 | 3 | Transferred to 54th Reg. P.V. July 4, 1864 Vet. |
| Barth, Frederick R. | do | June 18, '61 | 3 | Transferred to 54th Reg. P.V. July 4, 1864 Vet. |
| Brown, Lewis | do | June 18, '61 | 3 | Killed at Antietam, September 17, 1862 |
| Beidleman, Theo. | do | July 21, '61 | 3 | Deserted August 12, 1861 |
| Bushnell, Sam'l A. | do | May 27, '61 | 3 | Deserted March 22, 1864 returned April 8, '64 mustered out with company, June 17, 1864 |
| Crib, William | do | July 29, '61 | 3 | Discharged on Surgeons certificate Oct. 16, '62 |
| Crater, Jacob B. | do | July 29, '61 | 3 | Killed at Antietam, September 17, 1862 |
| Dougherty, Wm. | do | June 20, '61 | 3 | Absent, sick at muster out. |
| English, Emanuel | Private | July 20, '61 | 3 | Discharged on Surgeons certificate, Jan. 21, '63 |
| Evans, William D. | do | Sept. 1, '61 | 3 | Mustered out Sept. 4, 1864 expiration of term. |
| Flinn, Peter | do | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| Fill, John | do | June 29, '61 | 3 | Transferred to 54th regiment P.V. July 4, 1864. |
| Foll, John G. | do | June 18, '61 | 3 | Deserted September 24, 1862 |
| Gearhart, Abner C. | do | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| Greenwood, Lewis | do | Sept. 2, '62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Gerbron, Joseph | do | Sept. 2, '62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Gerlach, Charles | do | June 15, '61 | 3 | Deserted May 26, 1863 |
| Huff, Frederick | do | June 18, '61 | 3 | Mustered out with compnay, June 17, 1864 |
| Huver, James | do | July 20, '61 | 3 | Mustered out with company June 17, 1864 |
| Houseworth, Josiah | do | July 20, '61 | 3 | Mustered out with company, June 17, 1864 |
| Hertle, Michael | do | June 15, '61 | 3 | Absent, sick at muster out. |
| Hendricks, Hil'y O. | do | June 18, '61 | 3 | Discharged on Surgeons certificate Nov. 20, '62 |
| Hager, John S. | do | Oct. 10, '62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Huff, Andrew | do | June 29, 61 | 3 | Killed at Bull Run, August 30, 1862 |
| Jones, James | do | July 9, '61 | 3 | Disch. on Surg. certificate Dec. 2, '62 re-enlisted Mar. 31, '64 tr. to 54th reg. P.V. July 4, 1864 |
| Keeler, Joshua | do | June 18, '61 | 3 | Mustered out with company, July 17, 1864 |
| Kerns, Joseph | do | Aug. 6, '62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Keiser, Jesse | do | June 29, '61 | 3 | Died at Alexandria, Va. January 13, 1862 |
| Keiser, Adam | do | June 29, '61 | 3 | Killed at Gaines Mill June 27, 1862 |
| Ladshaw, Abram | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Lambenstine, John | do | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| Long, Washington | do | Oct. 28, '62 | 3 | Mustered out with company, June 17, 1864 |
| Longenbucher, Geo. | do | June 18, '61 | 3 | Died at Harrisburg, Pa. January 28, 1862 |
| Milloy, Daniel | do | Sept. 15, '62 | 3 | Discharged on Surgeons certificate Sept. 9, 1863 |
| Martin, John | do | Sept. 3, 62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Miller, Jeremiah | do | July 9, '61 | 3 | Deserted December 11, 1862 |
| Miller, William | do | June 18, '61 | 3 | Deserted December 22, 1862 |
| Ohl, John W. | do | Sept. 18, '62 | 3 | Discharged on Surgeons certificate, Jan. 18, '63 |
| Peters, Theodore C. | do | June 18, '61 | 3 | Discharged on Surgeons certificate, Feb. 11, '63 |
| Pennypacker, Jesse | do | Sept. 27, '62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Reed, James A. | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Reiter, Benjamin | do | June 18, '61 | 3 | Mustered out with company, June 17, 1864 |
| Reiter, George W. | do | June 29, '61 | 3 | Transferred to 54th regiment P.V. July 4, 1864 Vet. |
| Reed, Milton | do | Mar. 27, '62 | 3 | Transferred to 54th regiment P.V. July 4, 1864 |
| Roberts, A.J. | do | June 18, '61 | 3 | Killed at Bull Run August 30, 1862 |
| Reiter, George W. | do | July 29, '61 | 3 | Deserted December 29, 1863 |
| Rebman, George | do | Aug.30, '62 | 3 | Not on muster-out roll. |
| Scheetz, Milton | do | June 18, '61 | 3 | Mustered out with company June 17, 1864 |
| Small, John S. | do | June 29, '61 | 3 | Mustered out with comnpany June 17, 1864 |
| Seifert, Charles G. | do | June 18, '61 | 3 | Mustered out with company June 17, 1864 |
| Shively, John | do | July 16, '61 | 3 | Disch. on Surgeons cert. date unknown |
| Straun, Owen M. | do | Sept. 8, '62 | 3 | Transferred to Vet. Reserve Corps. July 29, 1863 |
| Shilb, Frederick | do | June 18, '61 | 3 | Transferred to 54th regiment P.V. July 4, 1864 Vet |
| Snable, Jeremiah | do | July 20, '61 | 3 | Transferred to 54th reg. P.V. July 4, 1864 Vet |
| Small, John | do | June 29, '61 | 3 | Transferred to western gun-boat service Feb. 28, 1862 |
| Schaarschmidt, E. | do | June 29, '61 | 3 | Deserted December 17, 1862 |
| Stouffer, Jacob L. | do | June 18, '61 | 3 | Missing in action at Gaines Mill June 27, 1862 |
| Sellers, Frank | do | June 18, '61 | 3 | Killed at Fredericksburg, December 13, 1862 |
| Thomas, George | do | June 18, '61 | 3 | Died at Washington, August 24, 1861 buried in Military Asylum Cemetery. |
| Trumbower, John | do | June 18, '61 | 3 | Transferred to 54th reg. P.V. July 4, 1864 |
| Trumbower, John | do | July 29, '61 | 3 | Deserted March 22, 1864 |
| Walp, Charles J. | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Wenkoldt, Jacob F. | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Wile, Michael | do | July 9, '61 | 3 | Mustered out with company June 17, 1864 |
| Willuner, Jesse | do | June 29, '61 | 3 | Mustered out with company June 17, 1864 |
| Weider, Wayne F. | do | June 18, '61 | 3 | Discharged on Surgeons certificate Mar. 5, '63 |
| Wissinger, Christ'n | do | June 18, '61 | 3 | Discharged on Surgeons certificate Feb. 11, '63 |
| Wissinger, Dan'l | do | June 18, '61 | 3 | Discharged on Surgeons certificate May 25, '63 |
| Warner, Stephen | do | July 20, '61 | 3 | Transferred to 54th reg. P.V. July 4, 1864 Vet |
| Worthington, George | do | June 18, '61 | 3 | Transferred to Signal Corps August 27, 1861 |
| Warner, Stephen | do | July 29, '61 | 3 | Deserted March 22, 1864 |
| Yeakel, John K. | do | Aug. 17, '62 | 3 | Transferred to Vet. Reserve Corps, July 29, 1863 |
| Yotter, Martin | do | June 29, '61 | 3 | Transferred to 54th reg. P.V. July 4, 1864 Vet |
| Yeakel, Samuel K. | do | Aug. 28, '62 | 3 | Transferred to 54th Reg. P.V. July 4, '64 |
| Zeiner, Levi | do | June 29, '61 | 3 | Discharged on Surgeons certificate Apr. 27, '62 |
| Zimmerman, Lewis | do | June 18, '61 | 3 | Transferred to 54th reg. P.V. July 4, '64 Vet. |
| Zimmerman, Lewis | do | July 29, '61 | 3 | Deserted March 22, 1864 |
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